Now, the Preds can enjoy a few days of rest while waiting to see what the second round holds in store. The tandem of David Legwand and Alexander Radulov led the way with a goal and an assist each, and Pekka Rinne made 21 saves, to finish the series with a sterling. The Wings are going to have to compete with both Nashville's physicality and skill, something they are more than capable of doing as long as their heads are in it.The Nashville Predators took a step forward as a franchise tonight, knocking off a Detroit Red Wings team which has, until now, always played King of the Hill against them. You can bet that they will come out hard and will do what they think it takes to win. The Predators are having a solid month so far and are fighting for a playoff spot. This is an important game for the Wings, who need to continue to improve their play as they see players return from injuries. Jeff (aka OklahomaWingNut) has his GDT up. Projected parings: Lidstrom-Rafalski Kronwall-Stuart Meech-Liljaįor more of the Wings' perspective see Snapshots, Abel to Yzerman, HockeyTownTodd, Behind the Jersey, No Pun Intended, Red Wings Nation, yzerman is god, Detroit Hockey, LetsGoWings, Red Wings Brasil, and Winging It In Motown. Projected lines: Filppula-Datsyuk-Hudler Franzen-Zetterberg-Samuelsson Maltby-Draper-Kopecky Drake-Hartigan-Downey The decision will be made following the pre-game skate. Nick Lidstrom (knee sprain) is expected to return, though the Freep lists him as questionable. Either Jonathan Ericsson or Derek Meech will fill in for him. Mark Hartigan will fill in as a result.Ĭhris Chelios (right leg, bone chip) is also out.īrett Lebda (illness) did not practice yesterday and will not play. The Wings will be without Holmstrom (groin/abdomen) today. Dan Cleary and Tomas Holmstrom, neither of whom are active today, both have 20. Zetterberg leads the team in goals with 36. Datsyuk has at least a point in each of his last five games.ĭatsyuk's 53 points are a team best. Henrik Zetterberg is not far behind with 76. Pavel Datsyuk is the leading scorer with 78 points. They have three games in hand on the Stars, who play the Avalanche again today at home. With Dallas' 3-1 loss in Colorado yesterday, the Wings maintain their seven-point lead over the League. That game kicked off a five-game homestand that continues this afternoon. They went on three nights later to defeat the St. The win was just their second in 11 games. David Legwand is questionable with a foot injury suffered in Calgary.įor the Predators' perspective, see Pred-Joe and On the Forecheck.ĭetroit (44-18-6) snapped a four-game losing streak with a 4-2 win in Buffalo on March 2nd. The Tennessean lists Steve Sullivan (back), Martin Gelinas (knee) and Jed Ortmeyer (knee) as out. Dumont has three goals over his past four games, including the overtime game-winner in Calgary. Martin Erat is the only other Nashville 20-goal scorer. Alexander Radulov, Martin Erat, and David Legwand each have 29 helpers.ĭumont is the leading goal-scorer with 26. Dumont (36) and Marek Zidlicky (35) are the other big assist men. JP Dumont is not far behind with 68.Īrnott's 39 assists are a team best. Jason Arnott leads the Predators with 63 points. A point today, though, would put them right back in the playoffs. Louis last night, the Predators fell from eighth to ninth place in the Conference. Today's game is the last of a six-game road trip that began in Buffalo on the 27th with an 8-4 loss. On the 7th, they defeated the Calgary Flames 2-1 in overtime. They then lost two nights later in Vancouver, 6-2. They also won their next game, a 5-1 decision over Edmonton on the 4th. The win snapped a three-game skid that began in the final week of February. Nashville (35-26-8) began March with a 3-1 win in Dallas. The teams meet again on March 15th, 20th, and 30th to wrap up the eight game regular season series. The Predators won November 22nd ( 3-2) and February 12th (4-2). The Wings won November 7th (3-2 SO) and December 10th (2-1). It is the fifth meeting between these two teams this season. The Detroit Red Wings host the Nashville Predators this afternoon in what could be a preview of the Western Conference Quarterfinals.
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SDS is a potent anionic detergent that coats denatured proteins with an equal anion to mass ratio this masks proteins' charge, shape, and size characteristics and renders them solely a function of molecular weight. Bromophenol blue (BPB) is a nonreactive reagent that serves as a dye front for electrophoresis. Glycerol adds density to samples, so they drift into the loading wells. Laemmli buffer (60mM Tris-HCl pH 6.8 20% glycerol 2% SDS 4% beta-mercaptoethanol 0.01% bromophenol blue) is unique to western blot sample preparation as each reagent is purposeful for SDS-PAGE. Therefore, there is always a 1 to 1 volume ratio of normalized protein and Laemmli buffer in a western blot sample. Protein extract gets normalized with cell-lysis buffer to the desired protein concentration, and there is an addition of an equal volume of Laemmli (sample) buffer. Thus, by running this assay with known protein standards, a linear regression standard curve is generated to calculate unknown protein extract concentrations.Īll western blot samples have three things: protein extract, cell lysis buffer, and Laemmli (sample) buffer. In brief, the dye Coomassie Brilliant Blue G-250 complexes with proteins to change color, and this absorbance shift gets recorded by a spectrophotometer. By conducting a Bradford assay, a colorimetric protein assay that exploits a dye’s interaction with protein, protein concentration is quantifiable. Intuitively, this is imperative for a valid experiment as unequal proteins per lane can skew the analysis. There must be an equal concentration of proteins per western blot sample. Collectively, this information underscores the need to tailor protein extraction to sample type and the target protein. PPIs are used to maintain the structure and phosphorylation status of the target protein from the activity of endogenous phosphatases upon cell lysis and exogenous phosphatases in the lysis microenvironment. As such, gentle buffers without detergents are required. Although rare, some antibodies will not be able to detect denatured samples. For example, radioimmunoprecipitation assay buffer (RIPA) is more adept for nuclear and mitochondrial proteins. Furthermore, the cell lysis buffer used in extraction should align with target protein cellular localization. For example, most tissue preparation is by homogenization or sonication however, osmotic shock or detergent lysis is more suited for easily lysed cells such as erythrocytes or cultured cells. There are numerous methods of extraction, and proper selection depends on the sample type. Western blot samples are first prepared by protein extraction with specialized cell lysis buffers and protease and phosphatase inhibitors (PPIs). Proper sample preparation for subsequent electrophoresis is crucial for downstream analysis. Western blot relies on the principles of equal loading of proteins, separation of proteins by molecular weight, electrophoretic transfer to a suitable membrane, and probing of antibodies. While Wallander’s personal life flounders after a recent divorce, he strives to find the killers of a well-respected elderly couple who owned a local farm. He divides his time between Sweden and Mozambique. Mankell was awarded the Crime Writers’ Association’s Macallan Gold Dagger and the German Tolerance Prize, among many others. It is the first book in the Wallander series, named after its recurring protagonist, a middle-aged detective based in Ystad, Sweden named Kurt Wallander. Henning Mankell's Kurt Wallander mysteries are global bestsellers and have been adapted for television as a BAFTA Awardwinning BBC series starring Kenneth Branagh. The woman supplies Wallander with his only clue: the perpetrators may have been foreign. Faceless Killers is a 1991 crime novel by Swedish author Henning Mankell. An old man has been tortured and beaten to death, his wife lies barely alive beside his shattered body, both victims of a violence beyond reason. He quickly becomes obsessed with solving the crime before the already tense situation explodes, but soon comes to realize that it will require all his reserves of energy and dedication to solve. When he reaches the isolated farmhouse he discovers a bloodbath. This is a writer who manages to convey massive talent and Faceless Killers is an incredible debut novel from writer who sadly is no longer with us. The main character of the story are Kurt Wallander. Faceless Killers pdf book was awarded with Glass Key Award (1992), Svenska Deckarakademins pris for basta svenska kriminalroman (1991). The book is perfect for those who wants to read mystery, crime books. Unlike the situation with his ex-wife, his estranged daughter, or the beautiful but married young prosecuter who has piqued his interest, in this case, Wallander finds a problem he can handle. Faceless Killers is a beautiful novel written by the famous author Henning Mankell. And as if this didn’t present enough problems for the Ystad police Inspector Kurt Wallander, the dying woman’s last word is foreign, leaving the police the one tangible clue they have–and in the process, the match that could inflame Sweden’s already smoldering anti-immigrant sentiments. And as if this didn’t present enough problems for the Ystad police Inspector Kurt Wallander, the dying woman’s last word is foreign, leaving the police the one tangible clue they have–and in th It was a senselessly violent crime: on a cold night in a remote Swedish farmhouse an elderly farmer is bludgeoned to death, and his wife is left to die with a noose around her neck. It was a senselessly violent crime: on a cold night in a remote Swedish farmhouse an elderly farmer is bludgeoned to death, and his wife is left to die with a noose around her neck. The Ruts: Babylon’s Burning (1979)īefore their career was tragically curtailed by singer Malcolm Owen’s premature death, highly proficient West London punks The Ruts looked like being one of the 80s’ major players. A highlight of their 1978 debut, First Issue, “Religion” was performed with a spine-chilling intensity and offered Lydon the opportunity to lambast church politics with gusto. Post-Sex Pistols, John Lydon formed the considerably more leftfield-inclined Public Image Limited (PiL). It remains a sinister but magnificent set piece. A scathing satirical attack on the then governor of California, Jerry Brown, vocalist Jello Biafra’s lyrics referred to a hippie-fascist US akin to the totalitarian regime imagined by George Orwell’s 1984. Influential San Franciscan punks Dead Kennedys’ brilliant debut single, “California Über Alles,” remains one of the best political punk songs to come out of the US. They could also knock out short, sharp and savage political punk with the best of ’em.Ĭlick to load video Dead Kennedys: California Über Alles (1978) Founders of the anarcho-punk subculture, the band’s music advocated animal rights, feminism and environmentalism, and sometimes leaned towards the avant-garde – as “Do They Owe Us A Living?” shows. Crass: Do They Owe Us A Living? (1978)Ī curious mix of hippie ideals and white-hot punk fury, Crass operated out of a communal house deep in Epping Forest, from where they promoted anarchism as both a political ideology and a resistance movement. However, they did write great songs full of socio-political commentary, also including “Guns Of Brixton” and the oil-crisis epic “Rock The Casbah.” Arguably better still is “Tommy Gun,” Joe Strummer’s hard-hitting critique of terrorists and the cult status their nefarious activities can attract. With hindsight, The Clash played the role of punk’s cultural ambassadors rather than nail their colors to any specific political mast. John Lydon’s furious invective still sends shivers up the spine, however, and, to this day, “God Save The Queen” packs an almighty clout that will forever ensure its place among the best political punk songs.Ĭlick to load video The Clash: Tommy Gun (1978) Sex Pistols: God Save The Queen (1977)Īrguably the daddy of all political punk songs, Sex Pistols’ notorious second single hijacked Queen Elizabeth II’s Silver Jubilee and shocked the public so much that Britain’s broadcasting bastion, the BBC, banned the song and refused to admit it had gone to No.1 during the summer of 1977. Listen to the best punk music on Spotify, and scroll down for our best political punk songs. Accordingly, punk has sired some of music’s most potent political critiques, and while few were written with longevity in mind, many of the best political punk songs have retained their relevance. Punk’s anti-establishment stance means it openly thrives on controversy and the desire to provoke, so its spearhead acts have inevitably been drawn to commenting on socio-political issues since the genre’s inception in the 70s. The Solitaire parade has one big inconvenience - it allows you to pay in gold for every new level you want to reach. Without knowing which new cards will appear in the process (maybe 6 cards on top of 8 cards, which will allow you to continue the sequence), the first variant seems to be a better choice. The sequence length you can make is shown below, so based on this evaluation, which one should you choose? By looking at the chart, you will see that the first sequence finally found three new cards (including three to four in the middle), while the second one only had two. The master card can be matched with 4 cards or 6 cards. Let's look at an example, as shown above. 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If you are using Maxthon or Brave as a browser, or have installed the Ghostery add-on, you should know that these programs send extra traffic to our servers for every page on the site that you browse.The most common causes of this issue are: Even without all of the add-on packs, the game has a ton to offer you and keep you busy for a while.Your IP address has been temporarily blocked due to a large number of HTTP requests. Of course, if you're buying the Sims 3, then you probably aren't too concerned about spending your time efficiently anyways.įinal thought: If you have the time (it's really addictive) then I would totally buy this game. You can just hop in the car and go anywhere in town freely without having to wait.Īnother thing is many people say it takes them a long time to sleep. There are no loading screens after your first initial load. The reason it takes so long to load is that it is essentially loading the entire town. But if you treat it right, it will perform great. If you are swinging the camera all over the place, jumping from one side of town to the other, or switching between build-mode and live mode constantly, then yes you'll probably have performance issues. But as for in-game performance, it really isn't all that bad, as long as you give it time to build up a cache. I heard it turns essentially into a slideshow. I heard that it would run incredibly slow on laptops. I have a dual core PC that is not made for gaming, and has a 3.4 Windows experience index. When doing the research for buying this game, I thought about what I would be running it on. The personalization of characters and their houses gives you so much freedom, not to mention all of the downloads out there offered by third party developers and websites. There is so much you can do within the game once you figure out how to work the control panels. Being completely new to the Sims, I figured I'd go with the newest, best one out there. The Sims 3 - Really not All That Bad on Laptopsįinding myself with a load of extra time on my hands, I bought the Sims 3. So it isn't hard to choose the expansions you want to play with. However, the icons are in the same order as on the Game Launcher, have the same colors, and the Game Launcher icons do have readable English pop-up text. The Sims 3 Addon loader (which selects which expansion and stuff packs are active) was probably originally run in Russian, because the pop-up boxes are largely unreadable (? in place of probably Cyrillic text). The game interface bar on the left is actually in English (probably set by the game setup). The Sims 3 Member login in the right section isn't needed to play the game. Minor annoyances (and workarounds) in the installation: the game launcher displays material in Russian on the right, even though the game setup specified English. Here is a report of issues with this download collection. There were several download options: Google Drive, OneDrive, and Torrent. The download was large, of course, some 16-18 GB of data. If you are familiar with Sims-type games, you have an idea what it's like. But on the one hand, it removed everything that made the franchise unique and overstuffed it with mediocre content that has pleased nobody. The combo channeling was completely eradicated leaving players to helplessly smash the same buttons endlessly. In addition, the humor wasn’t that great, and Yaiba is an easily forgotten character. Thankfully, it’s a spin-off and wasn’t developed by Team Ninja in the first place. Spark Unlimited was the big guy behind this mess, and no wonder they went bankrupt shortly after releasing it. Maybe if Yaiba wasn’t a Ninja Gaiden game, I feel that it would have performed well. Yaiba and DmC share the same relationship as being a forgettable experience. Ninja Gaiden Clans or known as 100万人のNINJA GAIDEN, 100 Man-ri no ninjagaiden, lit. NINJA GAIDEN of 1 Million People is a japan exclusive game for Android and iPhone devices. While the game borrows from the franchise, it is not considered canon. The gameplay is similar to Ninja Gaiden Dragon Sword, but it involves card collection trading. You attack your foes by swiping, and you can perform a Ninpo technique by following certain instructions. Other than that, there’s nothing special about this title.Ī North American release for the game was scheduled, but after a few months, the plan was unfortunately canceled. Ninja Gaiden 3 tried to cater to casual gamers who have had issues with the previous titles by simplifying the combat and making it unnecessarily edgier when that theme doesn’t fit a franchise such as Ninja Gaiden. Team Ninja sought to make Ninja Gaiden 3 an enjoyable experience, but they’ve ultimately failed at retaining the same momentum as its predecessors. Thank you again, Ashton, and I hope to see some of you over at The Domestic Rebel. And the frosting tastes exactly like rainbow sherbet, so you can just grab a spoon and have at it–I won't tell. I love the sweet and sour combo these cupcakes have! They're so tart and tangy yet sweet at the same time. I like the lightness soda adds to cake mix and the mixed berry flavor adds just a subtle sweetness to the cake that is so good. (nasdaq: pixy), a florida-based national staffing enterprise which designs, manages, and sells access to a disruptive, revolutionary platform that facilitates. One small straw (approximately 2.3 grams) contains about 9 calories. Nutrition Pixy Stix straws come in two sizes: small or large. The loose candy powder comes in a plastic wrapper designed to look like a drinking straw. How to make a Pixy Stick Drink - Pixy Stick Cocktail Recipe: Add ice to glass. **Note: if you can't find mixed berry 7-Up, substitute it with 12 ounces (1 can) of regular or cherry-flavored 7-Up. Pixy Stix is a popular candy made by the Willy Wonka Candy Company, a brand owned by Nestle USA. If desired, sprinkle frosted tops with more powdered drink mix or Pixie Stick powder for an extra pucker punch. Portion frosting among two piping bags and pipe orange frosting onto cooled cupcakes and top with cherry frosting. Investigation Takes a Turn A few days after Timothy was. The other contaminated sticks had enough to kill 34 adults in each candy. Stir half a packet of cherry drink mix into one bowl, and half a packet of orange drink mix into the other until combined. Timothy’s pixy stix contained enough cyanide to kill two adults. Divide frosting evenly among two bowls.Ģ. Gradually add powdered sugar, about one cup at a time, until light and fluffy. In the bowl of a stand mixer, beat together butter and lemon juice until creamy, about 1 minute. 14-16 minutes or until a toothpick inserted in the center comes out with light moist crumbs. Using a sharp knife, swirl the grape, berry blue and vanilla batters together very lightly. Then, top the white batter with one small teaspoon each of grape and berry blue batter. Put a heaping Tablespoon of white batter into each muffin tin. If desired, tint the batter with purple or blue food coloring to enhance the color.ģ. Stir in one packet of grape mix into one small bowl, and one packet of the berry blue mix into the other small bowl until each bowl is blended and flavored. Averigua lo que Pixy Stick (Pixystick) ha descubierto en Pinterest, la mayor colección de ideas del mundo. Spoon about 3/4 cup of batter into two separate small bowls (3/4 cup batter per bowl). In a large bowl, beat together the cake mix and 7-Up… that's it! No eggs, no oil–nothing! Just soda and mix. Line 2 muffin tins with about 15-18 paper liners and set aside.Ģ. Pass out these Pixy Stix picks at your next celebration These irresistible candy-filled straws are a flavorful way to fiesta when you add em to party. Pixie Frosting (recipe & ingredients follow)ġ.1 small envelope each cherry, orange, grape and berry blue (or tropical punch) Kool-Aid drink mix.However, I mastered the art of "smoking" them just like Big Michelle smoked her candy cigs. Step 1 - Rim a martini glass with grape Pixy Stix. Louis, MO 1940’s, they were sold as a drink mix. However, this is where my idolization stopped apparently because I wouldn't give up my Pixy Stix.even to be like Big Michelle. PIXI Formulado con ginseng, aloe vera y una mezcla única de extractos de frutas, este bálsamo teñido. When Pixy Sticks were invented by Sunline Inc. We found 19 other products Made By Sathers that either contain or have been processed in a facility with 19 other TOP 14 allergens. She always bought candy cigarettes and I thought she looked so cool when she "smoked" them. It looks like Sathers Candy Pixy Stix is Free From a total of: 6 different TOP 14 Allergens. She was 10 and at just 6 I longed for the double-digit days. The cool girl on the block was Big Michelle. Everyone had their favorite but my favorite, by far, was Pixy Stix. During those very long, hot summer days all the kids in the neighborhood would walk down to Mr. I was a child of the 70's and grew up in Hurst, Texas just three doors down from a Mr. In the 1950s the tasty powder was repackaged as Pixy Stix. Pixy Stix contents were originally sold as a drink mix in the 1940s but the manufacturer learned that kids were eating the sweet and sour powder right out of the package instead of mixing it with water. It is available on the newsstands in over 50 countries. Eye Spy is subscribed to by over 500 international government agencies and is used as an educational and training publication. This has enabled Eye Spy unique access to information often beyond the reach of media organisations. Through proper, non-political and unbiased reporting, he has secured the cooperation of many government offices, intelligence bodies, security services and military organs. His speciality lies in the working mechanism of the intelligence services and has extensive knowledge and liaisons with many government organisations, senior officials and former intelligence officers. He has worked as an investigative journalist, analyst and espionage writer for 35 years producing over 1,500 reports associated with the subject. If you have a broken frame and want to know if it can be fixed feel free to bring your frame to us or contact us on Twitter, Facebook, Google +, via our website or phone us at (403) 474-6744.Mark Birdsall is founder and editor of Eye Spy Intelligence Magazine - the world’s only independent newsstand publication devoted to intelligence and its numerous elements. We don't want to leave you high and dry (and visually impaired)! We will always work with you to find a solution no matter what the situation is and no matter where you bought the frame. There are going to be situations where you might come in with a frame you bought elsewhere or even one you got from us that cannot be repaired because the parts are no longer made. If you went to get same sweater there would be a good chance it's not made anymore). Often a frame that is only 1 or 2 years old is 'old' in the frame world and may have been discontinued (much like if you bought a sweater you really liked and two years later it ripped. It's also important to keep in mind that 'old' is relative. We don't carry discontinued frames and all of our frames come with at least a one year warranty for manufacturer's defects (meaning don't run it over with your car and expect the manufacturer to replace everything at no charge but anything reasonable is covered). Please don't do this. I would much rather you didn't have to pay for your repair and I bet you'd rather not pay either.Īt our office we really, really hate it when a frame breaks and we can't get parts anymore because it's been discontinued. If you have a warranty on your frame and you use super glue the warranty will be void. For a progressive (no line bifocal) it's essentially impossible to reuse the lens without cutting off the reading portion and having the lenses sit at the wrong height, angle and distance between the eyes resulting in terrible vision at all distances, distortion and eye strain. The frame chosen also needs to be the same size and shape or smaller in order to insert the lenses and this often restricts you to frames that don't actually fit you well. In the process of cutting a lens down and putting it into a new frame this is unlikely to line up properly and will lead to poor vision and eye strain. Lenses are set into your frame in a very specific way so that the optical centre of the lens lines up with your pupil. Sometimes when a frame breaks people wonder if they can use their same lenses in a new frame. We generally need to see the frame to know if we can do a quick repair, whether we need to order in parts or if the glasses are a lost cause.īefore I mention some common breaks and what we can and cannot do about them here are some things we cannot do and some things you shouldn't do. The first question everyone asks is "Can this be fixed?" The answer is frustrating because it really depends on what type of damage has happened. No matter how it happened you have an issue because you need those things to see. I always recommend that people have a back up or spare pair of glasses for situations like this but I know from personal experience that glasses seem to break at the worst possible time and usually when you don't have a spare pair. It's possible you stepped on them, they fell onto something hard, the dog ate them or they were just getting old and snapped. If you wear glasses chances are you've been in the nasty situation of having your glasses break. |
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